Reaction of 2, 4, 6-Trichiorophenyl 4-Aminophenyl Ether with Humic and Fulvic Acids in the Presences of Nitrite

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Addition of humic and fulvic acids to a mixture of 2, 4, 6-trichlorophenyl 4-aminophenyl ether (chloronitrofen-amino or CNP-amino) and nitrite resulted in an increase of coloration over a broad range of wave length. The increase of coloration was found in all preparations of soil humic and fulvic acids tested. CNP-amino was not recovered from the reaction mixture by alkaline treatment, but by Na2S2O4 reduction. The gel filtration of humic acid (Daisen) suggested that the humic acid could react with CNP-amino in nitrite solution, and became much larger molecules like humin.
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