Trace Determination of Phenols by High Performance Liquid Chromatography with an Electrochemical Detector:Pesticide Residue Analysis by High Performance Liquid Chromatography with an Electrochemical Detector (Part 2)
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We determined a trace amount of 14 phenols derived from pesticides by high performance liquid chromatography with an electrochemical detector. Phenols from aqueous and solid samples were enriched through exhaustive steam distillation and XAD-4 resin adsorption. Quantitative determinations were done by reverse-phase high performance liquid chromatography without derivatization. The minimum detectable quantity: phenol, 0.1ng; 2, 4-dichlorophenol, 0.4ng; 2, 4, 6-trichlorophenol, 0.4ng; 2-nitrophenol, 0.4ng. The recovery of phenols from egg plant samples fortified with each of 4-chlorophenol, 4-chloro-2-methylphenol and 2, 4-dichlorophenol at 1ppm ranged from 78 to 102%, while that of 4-nitro-phenol was poor. The recovery of 3-methyl-4-nitrophenol was not determined because other materials interfered.
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