Profiles of cytokine expression in radicular cyst-lining epithelium examined by RT-PCR.
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概要
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Levels of messenger RNA (mRNA) extracted from five samples of radicular cyst-lining epithelium were analyzed for cytokines, growth factors and epithelial cell growth-related receptors by RT-PCR. All five samples expressed IL-1α, -1β, IL-6, IL-8, IL-11, TGF-β1, PDGF-A and aFGF, and receptors for EGF (c-<I>erbB</I>), KGF, HGF (<I>c-met</I>) and IL-6. Some of the specimens expressed MIP-1α, RANTES, GM-CSF, M-CSF, TNF-α, PDGF-B and bFGF, but no expression of IL-2, IL-4, IFN-γ, IGF-I, EGF and KGF was detected. These results indicate that radicular cyst-lining epithelium, which is considered to be identical to the cell rests of Malassez, may play a role in periodontal pocket formation or apical cyst formation by interaction with surrounding connective tissue or hematopoietic cells through the expression of various cytokines. (J. Oral Sci. 42, 239-246, 2000)
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