Some Characters of Viruses Reconstituted with Components from Different Tobacco Mosaic Virus Strains

概要

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Mixed reconstitution was performed using the components of tobacco mosaic virus-ordinary strain (TMV-OM) and bean strain (TMV-B) under conditions of 0.1M pH 7.3 sodium pyrophosphate at 30C for 6-24hr. The recoveries of the reconstituted viruses, TMV-OM-RNA+TMV-B-protein and TMV-OM-protein+TMV-B-RNA, were 1.5-26.6% and both the viruses were infectious. Sucrose density gradient centrifugation of these 2 reconstituted viruses showed 2 peaks, both of which were infectious. The bottom peak sedimented near the position of the standard TMV, and its ratio of O. D. 260mμ/O. D. 280mμ was 1.22-1.29. The particles in this peak had the same length as that of the standard TMV. The top peak had the ratio of 1.28-1.41, and the length of particles in this peak were shorter than the standard TMV. The pattern of ECTEOLA-cellulose column chromatography of the reconstituted virus was similar to that of standard TMV. Further, it was confirmed that the biological characters of the reconstituted particles were the same as those of the RNA-donor TMV.
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