Study of the Method for Estimation of Digestibility by Lignin an Internal Marker in Roughages:—Recovery Rate of Modified Acid Detergent Lignin—

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Samples from two digestion trials in which three goats were fed Italian ryegrass hay or alfalfa hay ad libitum were used to study the analytical procedure of Acid Detergent Lignin (ADL) as an internal marker to estimate the digestibility of roughages. The results are as follows; 1. In preparation of samples for the ADL analysis, grinding with 0.5mm screen showed better result in accuracy than 1.0mm or 0.3mm. 2. Modified condition of digestion (time and temperature) by 72% H2SO4 did not improved the recovery rates of the ADL. 3. Prolonged extraction by acid detergent (AD) solution reduced the ADL contents; the lowest values were obtained in 4-10 hours of extraction in samples of digestion trial of Italian ryegrass hay and in 6-8 hours in Alfalfa hay. And the recovery rates of the ADL estimated from these lowest values were nearly close to 100%. 4. But the concentration of 1N H2SO4 of AD solution would be preferable, becouse high concentration of H2SO4 in AD solution caused to decrease ADL contents but not reduced the time of extraction by AD solution to obtained lowest ADL values. In conclusion, with the conventional analysis of ADL, if samples are ground with 0.5 mm screen and extracted by acid detergent solution for 6-8 hours, the ADL can be used as an internal marker to estimate the digestibility of roughages.
日本暖地畜産学会の論文