Analysis of the electrical charge of albumin in idiopathic nephrotic syndrome by isoelectric focusing.
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The state of the electrical charge of serum and urinary albumin was investigated in both the nephrotic and remission stage of idiopathic nephrotic syndrome (INS), using isoelectric focusing (IEF). 1) Both a b2 band (a main albumin band appearing at the site of isoelectric point:pI4.7), and a b3 band (a more anionic albumin band than the b2 band) were detected commonly in all samples of urine and serum of INS patients in nephrotic and remission stages and of healthy volunteer controls even if the applied albumin in urine and serum amounted to 20μg or 100μg. 2) When applied albumin amounted to 20μg, bl bands (less anionic albumin bands than the b2 band) were detected between pI 4.7 and p16.5 in urine and in both serum and purified albumin fractionated from serum of INS patients in the nephrotic stage. However, b1 bands were not detected at all in the urine and serum of either INS patients in remission stage and healthy volunteer controls. 3) When applied albumin amounted to 100μg, bl bands were detected also in serum of healthy volunteer controls. From these results, it was confirmed that less anionic albumin existed also in the serum of healthy volunteer controls although the amount was extremely small. Moreover, it was suggested that less anionic albumin appeared in greater quantity in the serum of INS patients in the nephrotic stage than in serum of healthy volunteer controls, and that this increased amount of less anionic albumin would leak more easily into urine through the anionic charge barrier of the glomerular basement membrane than the main albumin (b2 and b3 bands) would.
- 社団法人 日本腎臓学会の論文
社団法人 日本腎臓学会 | 論文
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