Effect of uremic middle molecules removed during hemofiltration on the enzyme activity in rat liver cytosol.

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It is well known that the products of protein catabolism and some of the intermediates including middle molecules (MM) are a biochemical feature of uremic serum and also the activity of serum alanine aminotransferase decreases relatively. To examine the toxicity of MM, we investigate the effect of MM removed by HF on aminotransf erases and peptidases in rat liver cytosol. The following enzymes were selected: aminotransf erases (GOT, GPT), leucine aminopeptidase (LAP), γ-glutamyltranspeptidase (γGTP). MM samples were as follows: preparation of MM fraction from each 10l HF fluid at half intervals of former (F-) and latter (L-) for 5h. HF of two patients (5 weeks) by 1KD ultrafiltration, and group separation into hydrophobic (Mo) and hydrophilic (Mi) by XAD-4 resin (F-Mo, L-Mo, F-Mi, L-Mi). Except for GOT, the effect of MM were found at the activities decreased on LAP (26-30%), on GPT (5-6%), and increased on γGTP (4-23%), as compared to control. We found a little difference in the results by the character of MM (Mo, Mi) and by the intervals of HF (F-, L-). These results suggest that MM might play a role in the formation of uremic peptides.
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