Probrems in serum aluminum determination by atomic absorption spectrometry.
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概要
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Electrothermal atomic absorption spectrophotometry has been used to analyze aluminum in various kinds of materials. However, several technical problems has to be solved to apply this method on determining aluminum in biological materials, including instrument setting, standardization procedure and eliminating contamination. This method consists of drying, ashing and atomizing material, all of which are performed by graphite furnace. Reproducibility of measurement largely depends on the steps of drying and atomizing. Interferences could be elininated to a great degree in ashing step when the temperature was above 1100°C and ashing time was 20 seconds. In order to eliminate or to reduce background of measurement and chemical interferences, matrix modifiers have been used in atomic absorption spectrophotometry. We employed serum based standard method for serum sample to avoid using multiple kinds of modifirs which causes high reagent blank. In this method, reagent blank has not been detected. Precision of this method whithin-and between-assay were CV=2.7% and 4.2%, respectively. Mean recovery of externally added aluminum was 101.8%. We also investigated the possible sources of aluminum comtamination. In this study, we succeeded to establish highly accurate and reliable method for measuring serum aluminum concentration. This technology enables us to make an accurate diagnosis on aluminum toxicity in the patients with chronic renal failure.
- 社団法人 日本腎臓学会の論文
社団法人 日本腎臓学会 | 論文
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