Total Protein Concentrations and Protein Fractions in Whole Saliva from Healthy Subjects and Edentulous Subjects, Measured by Cellulose Acetate Membrane Electrophoresis and High Sensitivity Silver Staining.
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Whole saliva was collected by a spit out method from 52 dentulous subjects (21 to 31 years old) who had no systemic disease and were diagnosed to have normal periodontium by oral cavity examination and from 11 subjects (60 to 85 years old) who were completely edentulous and using complete dentures, and the concentration of total protein and protein fractions in collected whole saliva were measured using cellulose acetate membrane and the silver staining method developed by Shiba et al. Since this silver staining method is highly sensitive, prior concentration of saliva was not needed.<BR>The mean ± S.D. of the total protein concentrations obtained by dot blotting-silver staining was 1, 197.3 ± 698.5 mg/<I>l</I> for the group of all dentulous subjects and 2, 202.0 ±1, 625.0 mg/<I>l</I> for the group of all edentulous subjects, and these values were significantly different (<I>p</I><0.05) . Sex difference was not significant in either group.<BR>In protein fractionation by means of cellulose acetate membrane electrophoresis, the following 8 protein fractions could be fractionat-ed clearly: (from the anode) albumin, pre-α<SUB>1</SUB>-globulin, α<SUB>1</SUB>-globulin, α<SUB>2</SUB>-globulin, β<SUB>1</SUB>-globulin, β<SUB>2</SUB>-globulin, IgA and γ-globulin. In all specimens analyzed, IgA and albumin fractions showed sharp peaks. The IgA fraction had the largest percentage area among the 8 protein fractions, which was 36.7 ± 5.6% (mean t S.D.) in dentulous subjects and 39.0 ± 4.6% in edentulous subjects. The albumin and β<SUB>1</SUB>-globulin fractions showed significantly greater percentage areas in dentulous subjects than in edentulous subjects, whereas the percentage area of γ-globulin fraction was significantly greater in edentulous subjects (<I>p</I><0.01) . The concentration of individual proteins, which was calculated by multiplying the total protein concentration to the percentage fraction. Edentulous subjects showed significantly greater concentrations of IgA and γ-globulin (<I>p</I>< 0.05) . No sex difference was observed in the percentage area and the concentration of individual protein fractions in either group. Among salivary proteins, IgA and albumin were identified by immunofixation.
- 一般社団法人 日本総合健診医学会の論文
一般社団法人 日本総合健診医学会 | 論文
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