A Study on Preincubation Condition for the Microsuspension Assay.
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概要
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Effect of preincubation time and density of bacterial suspension on the sensitivity of Kado's microsuspension assay (modified liquid incubation assay) were studied by using <I>Salmonella typhimurium</I> TA100 and extract of airborne particles.<BR>Detection sensitivity increased in proportion to the cell concentration in the range from 1.3×10<SUP>8</SUP> cells/ml to 1.4×10<SUP>10</SUP> cells/ml. It showed 7 times higher sensitivity than that of the lowest density (1.3×10<SUP>8</SUP> cells/ml) when a high cell density (1.1×10<SUP>10</SUP> cells/ml) was used. Prolonged preincubation (50-140 min) increased the sensitivity at a higher cell density. At the highest cell density employed, cell growth was suppressed probably due to evaporation of water during the preincubation. Employment of seal to the microvial showed the good result. These modifications gave us more sensitive and useful mutagenic test with very small amount sample.
- 日本環境化学会の論文
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