Mutagenicity of 12 HPLC Labeling Reagents for Analysing Carboxyl Compounds.

概要

論文の詳細を見る
Mutagenic activity of 12 HPLC labeling reagents, which are widely used in analytical laboratories, were assessed by the preincubation assay using Salmonella typhimurium TA98 and TA 100 under condition with or without metabolic activation by S9mix. The compounds tested were : 1-benzyl-3-p-tolyltriazene [BTT], 1-p-nitrobenzyl-3-p-tolyltriazene [NBTT], N-chloromethylphthalimide [CMP], N-chloromethyl-4-nitrophthalimide [CMNP], 9-chloromethylanthracene [CMA], O- (p-nitrobenzyl) -N, N'-diisopropylisourea [NBDI], 7-acetoxy-4-bromomethylcoumarin [BrMAC], 4-bromomethyl-7-methoxycoumarin [BrMMC], phenacyl bromide [PB], p-phenylphenacyl bromide [PPB], p-bromophenacyl bromide [BPB] and m-methoxyphenacyl bromide [MPB] . They are all used as the labeling reagents for detecting carboxylic acids by HPLC. Among these compounds, six compounds, BTT, NBTT, CMP, CMNP, CMA and NBDI showed high mutagenicity except in the case of NBDI with TA98. These chemicals showed higher mutagenicity for TA100 than TA98. BTT, NBTT and CMA showed higher mutagenicity without S9mix than with S9mix. Four kinds of phenacyl bromides (PBs) dissolved in DMSO showed mutagenicity against TA 100 without S9mix, though these compounds dissolved in acetone did not. It was suggested that the PBs dissolved in DMSO were oxidized to be mutagenic phenylglyoxals. No mutagenicity was observed by 2 coumarins reagents.
日本環境化学会の論文