A Test for Detecting Trace of Organophosphorus Pesticides in Environment by Enzyme Inhibiting Assay: Sample Preparation Method.

概要

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A reproducible method, based on the chemical oxidation and cholinesterase (ChE) inhibition assay, is described for monitoring trace of organophosphorus pesticides in aqueous samples. Chlorine was tested as oxidizing reagent for conversion of various thiophosphorus pesticides (P=S compounds) into thier P=O analogs having higher ChE inhibiting activity. After treatment with chlorine (10mg/L) of buffered pesticide solutions (pH 6.0) at 20°C for 15 min, ChE inhibiting activities and detection limits for each pesticide solution were determined. Greater ChE inhibiting activities, leading to the lower detection limits (ppb levels), were observed for chlorine-treated solutions fortified daizinon, EPN, parathion and fenitrothion and etc. This combination of oxidative derivatization and ChE inhibition assay was applied to the detection and determination of parathion, diazinon, and fenitrothion in natural water, soil, and vegetable samples at 1-300 ppb fortification levels.
日本環境化学会の論文