Assessment of the PCNA-Positive Cells of Hematopoietic Progenitor Cells in Umbilical Cord Blood.
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概要
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Proliferating cell nuclear antigen (PCNA) is a nuclear protein associated with the cell cycle. Nuclear PCNA immunoreactivity is found in the proliferative compartment of normal tissues and cells. In these studies, there has been a positive linear relationship between the number of cells with PCNA expression and the number of S-G<SUB>2</SUB>/M phase cells in phytohemagglutinin-stimulated umbilical cord blood mononuclear cells. Human umbilical cord blood mononuclear cells and CD34 cells which are semi-purified hematopoietic progenitor cells showed only very rare (<5%) PCNA immunoreactive cells. After phytohemagglutinin stimulation, PCNA-positive cells were increased in the majority of cells by 72 hours. PCNA-positive cells of cord blood mononuclear cells and CD34-positive cells were increased during 7 to 10 culture days when these cell were stimulated by rhIL-3. Nuclear PCNA immunoreactivity has been expressed also in the proliferative compartment of hematopoietic stem cells. These results appear to be a new method enabling analysis of the proliferative compartment of hematopoietic stem cells.
- 特定非営利活動法人 日本小児血液・がん学会の論文
特定非営利活動法人 日本小児血液・がん学会 | 論文
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