Cell Surface Analysis of Leukocytes in Cerebrospinal Fluid by Indirect Immune-Rosette Method. Identification of Central Nervous System Infiltration of Acute Lymphoblastic Leukemia and Non Hodgkin's Lymphoma.:Identification of Central Nervous System Infilt

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By means of a novel method, indirect immune-rosette method, leukocytes obtained from cerebrospinal fluid (CSF) in patients, six common acute lymphoblastic leukemia (ALL), one T-ALL and one T-non Hodgkin's lymphoma, who exhibited pleocytosis during complete remission were evaluated for cell surface antigen expression, together with ten aseptic meningitis with no malignancy as a control. In six out of the eight patients, antigen expression patterns of leukocytes in CSF were coincident with that of lymphoblasts at onset. In these cases cell number was promptly reduced after intrathecal administration of methotrexate. In the remaining two patients, the leukocytes in CSF demonstrated different patterns from those of the lymphoblasts at onset, and they were eventually diagnosed as having aseptic meningitis by their clinical course. The leukocytes in CSF of the two patients revealed multi-clone pattern with CD2, CD3, CD13 and CD14 positive which was exactly identical to that of aseptic meningitis of the control. This indirect immune-rosette method has a great advantage for identification of central nervous system infiltration of malignant cells or viral infection, in that a small number of cells, approximately 100 cells for one antibody respectively, is sufficient for each antigen analysis.
特定非営利活動法人　日本小児血液・がん学会の論文