埼玉県における下痢症患者から分離された腸管出血性大腸菌の細菌学的性状について(1990〜1992年)

概要

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A total of 16 strains of Enterohemorrhagic Escherichia coli (EHEC) isolated from diarrea patients in Saitama from 1990 to 1992 were tested for their serotype, verotoxin production, biochemical characteristics, antibiotics sensitivity and plasmid profiles.<BR>By serotype analysis, 14 strains from two outbreaks and 12 sporadic cases were classified as type 0157: H7, one as O111: H-(not motility) and one as 0128: H2. Typing of verotoxin by gene analysis using Polymerase Chain Reaction (PCR) showed that 9 of 0157: H7 strains including two cases from outbreaks and O128: H2 have VT1 and VT2 genes, other 0157: H7 have the VT2 gene and 0111: H-has only the VT1 gene. Biochemical characteristic anlaysis indicated twostrains of 0157: H7 type from outbreaks were biotype II and the rest of 0157: H7 wre biotype I.<BR>One of the 0157: H7 strain from a sporadic case showed positive for urease production.<BR>According to sensitivity tests against antibiotics, out of the 0157: H7 group, one strain was resistant against ABPC, one against SM and two strains resistant to SM-TC.<BR>For plasmid profiles, all strains had 94 Kb plasmids and several smaller sizes of plasmids. But 5 strains of 0157: H7 had 94 Kb plasmid only.
日本感染症学会の論文