溶連菌の群別,およびA群溶連菌の型別-1-Electrosyneresis法による溶連菌の群別,およびA群溶連菌の型別について (猩紅熱研究会年次報告研究資料集--1974年)

概要

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Studies were carried out to develop and evaluate a electrosyneresis for the rapid, sensitive, and specific grouping of beta hemolytic streptococci and typing of group A hemolytic streptococci. In grouping and typing of strep ococci, well defined and centrally loca ed precipitin bands were produced by using a gel composed of Special Agar Noble: Agarose A-45=1: 2. Electrophoresis was carried out for 20 min. by using 9 mA/cm at room temperature with group specific or type specific antiserum. The precipitin bands were detectable during the electrophoresis. In such conditions, 1.7 μg/ml of purified group A polysaccharide could be detected.<BR>When a antiserum well was placed opposite to two antigen wells, group and types of group A streptococci were identified at a same time with non-absorbed respective type of group A antiserum.
日本感染症学会の論文