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Immunomagnetic separation technique was used for the isolation of Salmonella from 87 raw chicken meat specimens. Two kinds of immunomagnetic beads are available in Japan. Both of them were covered with Salmonella antibodies, but one was for O-antigens and the other was for H-antigens. In this study, two kinds of immunomagnetic beads were mixed and added to the broth for isolation testing. Findings were compared with those obtained by the single use of immunomagnetic beads and the conventional method. The mixed immunomagnetic beads isolated 15 of 17 positive samples, 13 by beads for Oantigens, 11 by conventional method, and 10 by beads for H-antigens. The isolation rate was increased by using both types of immunomagnetic beads because their targets were different. The mixed immunomagnetic beads enhanced the likelihood of isolating Salmonella.
日本食品微生物学会の論文