Cyclopiazonic acid discharges intracellular Ca2+ stores and stimulates Ca2+ influx in cultured human gingival fibroblasts

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The effect of cyclopiazonic acid (CPA) on changes in the intracellular free Ca2+ concentration ([Ca2+] i) evoked by bradykinin (BK), histamine (HIST) and thapsigargin (TG) was investigated in human gingival fibroblasts.CPA itself dose-dependently stimulated [Ca2+] i responses in both the absence and presence of extracellular Ca2+. Pretreatment with CPA (<5μM) enhanced the [Ca2+] i responses evoked by 5 nM BK and 1 mM HIST. However, CPA-pretreatment depressed the [Ca2+] i response evoked by 1μM TG in a dose-dependent manner. Moreover, CPA accelerated the Ca2+influx caused by 5 nM BK and 1mM HIST, but did not alter that caused by 1μM TG. These results indicate that CPA discharges intracellular Ca2+stores, resulting in their depletion, and enhances Ca2+influx across the plasma membrane. (J.Oral Sci.45, 139-144, 2003)
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