Hepatocyte growth factor(HGF) system in gingiva: HGF activator expression by gingival epithelial cells.
スポンサーリンク
概要
- 論文の詳細を見る
Hepatocyte growth factor (HGF) acts as a mitogen, motogen, morphogen, and anti-apoptotic factor for various kinds of epithelial cells. We previously showed that periodontal ligament and gingival fibroblasts secreted an HGF-like chemoattractant for a gingival epithelial cell line and found that the HGF content of gingival crevicular fluid was well correlated with clinical parameters and interleukin-1β level. Since HGF is secreted as an inactive form (proHGF), and converted to an active form by serine proteases such as HGF activator (HGFA), extracellular processing of proHGF is presumed to be critical in the regulation of HGF activity. To examine the role of the HGF system in epithelial invasion followed by loss of connective tissue attachment in periodontitis, mRNA expression of HGF, its receptor (<I>c-met</I>) and HGFA in gingival tissues was monitored. Ten gingival biopsies were obtained, and epithelium and connective tissues were separated by enzymatic digestion. The gene expression of HGF and keratinocyte growth factor (KGF) in gingival connective tissue, and<I>c-met</I>, HGFA and KGF receptor (KGFR) in gingival epithelial tissues was monitored using RTPCR. Furthermore, HGFA protein in the conditioned medium of cultured primary gingival epithelial cells was examined using Western blotting. All the connective tissue samples expressed KGF, and 8 out of 10 samples expressed HGF. All the epithelial samples expressed KGFR and<I>c-met</I>, whereas 5 out of 10 samples expressed HGFA. Protein expression of HGFA by cultured primary gingival epithelial cells was also confirmed. In terms of local production and activation of HGF in gingival tissue, these results suggest that synergistic expression of HGF in connective tissue and HGFA expression in epithelium may contribute to disease progression in periodontitis. (J. Oral Sci. 44, 129-134, 2002)
- 日本大学歯学部の論文
日本大学歯学部 | 論文
- Comparative studies on the effect of crude aqueous (CA) and solvent (CM) extracts of clove on the cariogenic properties of Streptococcus mutans
- Effect of preliminary irradiation on depth of cure and hardness of a veneering composite
- Bond strength to bovine dentin of a composite core build-up material combined with four different bonding agents
- Depth of cure and hardness of an indirect composite polymerized with three laboratory curing units
- Effects of alumina air-abrasion and acidic priming agents on bonding between SUS XM27 steel and auto-polymerizing acrylic resin