Synchronous Cell Differentiation in Caulobacter crescentus

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概要

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• The growth of a stalked bacterium, Caulobactei crescentus, has been synchronized easily and repro-ducibly by a new method. When this bacterium is grown to a late log phase in nutrient broth at 30 C with aeration, swarmer cells are accumulated in the culture to 80% of the whole cell population. When this culture is inoculated into fresh pre-warmed broth at twentyfold dilution, it immediately initiates synchronous cell growth. Simultaneously, synchronous cell differentiation is monitored by the sus-ceptibility of the cells to RNA phage infection. The swarmer cells accumulated in the late log phase of growth possess nearly the same susceptibility to RNA phage infection as those in the early log phase of growth while RNA phage-adsorbing capacity is lower in such swarmer cells. It is suggested that the swarmer cells accumulated in the late log phase of growth have lost some pili.

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著者

• OKADA Yoshimi

  Deportment of Biophysics and Biochemistry, Faculty of Sciences, University of Tokyo

• IBA Hideo

  Deportment of Biophysics and Biochemistry, Faculty of Sciences, University of Tokyo

• FUKUDA Akio

  Deportment of Biophysics and Biochemistry, Faculty of Sciences, University of Tokyo

関連論文

• Synchronous Cell Differentiation in Caulobacter crescentus

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