Mutagenicity Detection Method for Gaseous Compounds in Tobacco Smoke using Plural Impinging Exposure System.

概要

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A simple mutagenicity detection method for gaseous compounds in tobacco smoke was examined as a part of study for the evaluation of mutagenicity of air pollutants. A main stream and a side stream of tobacco smoke were introduced separately to impinging tubes containing bacterial solutions and bubbled them. The mutagenicity was estimated by the umu test using <I>Salmonella typhimurium</I>OY413 and TA1535/pSK1002 strains. The enzymatic activity obtained by the umu test was corrected with the survival rate of the tester strains which was evaluated by testing the strains to hydrogen peroxide. The correction by the plate spread method gave more exact mutagenicity than the conventional correction with absorbance at a wavelength of600 nm. For the detection of the mutagenicity of the gaseous compounds in the tobacco smoke, it was required to connect 6 impinging tubes in a series. The gaseous compounds showed lower mutagenicity than the particles containing in the tobacco smoke both for the main stream and the side stream of the tobacco smoke.
日本環境化学会の論文