Microbial Degradation of Polycyclic Aromatic Hydrocarbons.
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概要
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Strain H2-5, a gram positive and rod bacterium which was capable of degrading pyrene, was isolated from soil. The bacterium was positive for acid-fast staining. The optimum growth temperature was 34.3°C, and the upper limit temperature for the growth was around 45°C. At low concentration (1.3 μg/ml) of polycyclic aromatic hydrocarbons (PAHs), H2-5 cells (45μg dry weight/ml) grown on TSB degraded 90% of pyrene in 12 hr, and degraded 60% of Benzo (a) pyrene (BaP), 25% of Benz (a) anthracene (BaA), and 8% of Benzo (ghi) perylene (BghiP) in 3 days. At high concentration (26.7μg/ml) of PAHs, H2-5 cells (226μg dry weight ml) grown on TSB degraded more than 80% of pyrene and BaP, 40% of BaA, and 30% of BghiP in 11 days. Mixture of cells (5.4μg dry weight/ml) grown on pyrene-enriched culture, from which H2-5 was isolated, degraded pyrene more rapidly at early reaction stage, but less effectively at later stage, than the isolated H2-5 (226μg dry weight ml) grown on TSB, and both degraded 90% of pyrene in 11 days.
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日本環境化学会 | 論文
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