Improvement of the Sample Preparation Method for the Rapid Analysis of Thianphenicol in Meat and Fish.
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概要
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Thianphenicol is one of the anti-bacterials for domesutic animals and fishes. The investigation into the residual anti-bacterials in meat is necessary for public health. In the official method, thianphenicol extracted from meat is analyzed using HPLC/UV absorption at 230nm. However, some compounds interfere with the analysis when the antibacterial in internal organs such as kidney is analyzed. In order to remove the interfering compounds, we improved the preparation method for thianphenicol by modification of the previous method (Kiuchi and Fujimoto, 1991) . The improved method was performed by delipidation with n-hexane, extraction with acetonitril and fractionation by silicic acid column chromatography. The recovery of thianphenicol added to 1 μg/5g hog kidney homogenate, was 70.1%.
- 日本環境化学会の論文
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