Modification of umu Test Using the Bioluminescent Bacteria and Application to Sediments and Soils.

概要

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A type of umu test method that utilizes new bioluminescent bacteria has been developed. The method does not use any reagents and is able to simultaneously examine many samples with a 96-well plate. Thus the method is simple compared with the conventional umu test. The bacteria used for the method is <I>Salmonella typhimurium</I> TA1535/pTL210 that was transformed to generate luminescence induced by the expression of SOS gene caused by DNA damaging agents. As a result of examinations for the best reaction conditions, the highest and the most stable intensity of bioluminescence was obtained at the reaction conditions of 30°C and 180-240 min. From the results of comparison with the conventional umu test using 9 kinds of typical mutagens, the method showed satisfactory dose-response curves and its sensitivities for the test compounds were 1.6 to 33 times higher than those of the conventional umu test. In addition, in order to confirm the applicability to actual environmental samples, extracts of sea sediments and soils were examined by the method with or without S9. All of them showed genotoxicity only with S9. Their intensities of genotoxicity corresponded with total concentrations of chemicals that were determined by GC/MS. From these results, it was confirmed that the method is useful for evaluating genotoxicity in environmental samples.
日本環境化学会の論文