Bactericidal Effect of Alkalic Electrolyzed Water.
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概要
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Aqua oxidized water (AOW) and Aqua alkalic water (AAW) are made by acidic electrolyzed water making equipment; OXILIZER (OXILIZER Co., Tokyo, Japan) . AOW is acidic water with a pH under 2.7 and is processed by electrolysis through a small NaCl mediated specific membrane. It was over 1, 100mV of oxidation-reduction potential and includes Cl<SUB>2</SUB>, OH⋅, O<SUB>3</SUB>, ClO. AAW is alkalic water with pH 11.<BR>AOW has a strong bactericidal effect on bacteria and virus but the effect is reduced by the large number of organisms. It has a metallic corrosive function as well as an organic matter action. There are few reports about AAW sterilizing without a metallic corrosive action. The bactericidal effect of AAW to periodontopathic bacteria [<I>Actinobacillus actinomycetemcomitans</I> (Aa), <I>Porphyromonas gingivalis</I> (Pg), <I>Prevotella intermedia</I> (Pi), <I>Fusobacterium nucleatum</I> (Fn) ] and <I>Staphylococcus aureus</I> (Sa) and <I>Escherichia coli</I> (Ec) was investigated. The bactericidal effect of AOW and AAW were compared.<BR>Bacterial strain Sa : 209-P, Ec : B, Aa : Y4 and ATCC 29522, Pg : 381 and ATCC 33277, Pi : 25611, Fn : 25586 were grown at 3TC in BHI (aerobic and anaerobic condition), GAM contained hemin, menadion and yeast (anaerobic condition), and GAM broth (anaerobic condition), respectively. After washing by centrifugation, each bacteria was suspended in PBS.<BR>Serum and saliva were obtained from one healthy volunteer.<BR>Bactericidal activity was measured by colony count seeding and grown on BHI or Anaero columbia blood agar or GAM medium after treatment with AAW or AOW.<BR>The results were as follows:<BR>1. Bactericidal effect and effective concentration of AAW<BR>Aa, Pg, Pi were killed in one minute (Aa; 10<SUP>8</SUP> to 10<SUP>4</SUP> CFU/m<I>l</I>, Pg; 10<SUP>8</SUP> to 10<SUP>3</SUP> CFU/m<I>l</I>, Pi ; less than 10<SUP>8</SUP> to 10<SUP>3</SUP> CFU/m<I>l</I>) from original 100% AAW, while it took more than one minute to kill Fn, and Sa and Ec had survived after ten minutes. Further, sterilizing activity decreased with AAW' s dilution. AOW shows that a 25% concentration is most effective against periodontopathic bacteria.<BR>2. The effect of saliva and serum on bactericidal activity of AAW<BR>As for Aa, Pg and Pi, the addition of saliva or serum in a concentration of 1% of AAW had decreased bactericidal activity slightly. However, the bactericidal activity was completely negated by the addition of 10% serum of AAW. As for Fn, the bactericidal activity was negated in 1% of serumor salivery addition.
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