Role of FGF-2 in interaction of oral squamous cell carcinoma cells and fibroblasts in an in vitro invasion system
スポンサーリンク
概要
- 論文の詳細を見る
Stromal fibrosis occurs as a host reaction to invasion and proliferation of cancer cells, and cancer cells are activated through their interaction with proliferating fibroblasts. However, the detailed mechanism remains to be clarified. In this study, we investigated the influence of FGF-2, which is excessively expressed in cancer cells at the invasive front, on the proliferation of fibroblasts and invasion and proliferation of cancer cells <I>in vitro</I>. The culture supernatant of three cell strains of human oral squamous cell carcinoma (OSC-20: type 3; OSC-19: type 4C; and HOC313: type 4D), among which the mode of cancer invasion differed, promoted proliferation of human normal gingival fibroblasts (HGF-1) . In particular, the culture supernatant of HOC313 cells markedly promoted proliferation of HGF-1 cells, and FGF-2 expression in the supernatant was more marked than in the culture supernatant of OSC-20 and OSC-19 cells. Furthermore, Recombinant Human FGF-2 (50ng/ml) promoted not only proliferation of HGF-1 cells but also invasion and proliferation of each cancer cell, and the levels of OSC-19 and HOC313 cells were significantly higher than the control values (p<0.05) . In an <I>in vitro</I> invasion model, addition of an FGF-2-neutralizing antibody (2μg/ml) inhibited invasion of OSC-20 and OSC-19 cells. These results suggest that highly invasive squamous cell carcinoma cells produce FGF-2 and promote invasion and proliferation activity of cancer cells via autocrine, whereas it is involved in the proliferation of fibroblasts via paracrine.
- 一般社団法人 日本口腔腫瘍学会の論文
一般社団法人 日本口腔腫瘍学会 | 論文
- 進行口腔癌に対する逆行性超選択的動注化学療法, 放射線同時併用療法後の救済手術
- TS-1による化学療法と温熱放射線療法が著効した後発頸部リンパ節転移の1例
- 手術シミュレーションに基づく腫瘍分割摘出により顎骨を保存した得た下顎骨骨形成線維腫の1例
- 口腔癌における化学・放射線療法後の再発・二次再建に関する外科療法の検討
- 下顎骨再建 : 血管柄付遊離骨移植と遊離軟部組織移植の比較