Cell kinetic analysis of oral squamous cell carcinoma by using nuclear DNA-RNA contents and antibody to bromodeoxyuridine.

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Concerning cell kinetics of oral cancer, the following findings were obtained as a result of both measurement of nuclear DNA-RNA contents, and examination using antiBromodeoxyuridine (BrdU) monoclonal antibody.<BR>1. A significant difference was noted between the BrdU labelling index of normal gingival epithelium (2.9±0.5%) and that of oral squamous cell carcinoma (11.4±3.8%) . In the normal epithelium positive identification was restricted to the basal cell layer or the 2nd-3rd supra basal cell layers ; while in squamous cell carcinoma, it was commonly observed to occur around the focus of tumor cells in the well differentiated area, and was seen scattered in the poorly differentiated area.<BR>2. No definite relationship was observed between the BrdU labelling index and clinical stages of tumor. With respect to the relationship with histopathological malignancy, the labelling index did not show any association with the grading of differentiation or atypia, but showed positive correlation with the mitotic index. The value of the labelling index was 11.5 times as large as that of mitotic index.<BR>3. With the introduction of the BrdU labelling index applied to the results of measurement of nuclear DNA-RNA contents was it admittedly possible to clarify its significance in oral squamous cell carcinoma, a factor which had not been revealed by conventional analyses.
一般社団法人日本口腔腫瘍学会の論文