Osteoblast-Related Gene Expression of Rat Bone Marrow Cells Induced by Three-dimensional Cell Culture in Type I Collagen Gel.

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Bone marrow stromal cells contain multipotent stem cells that differentiate into several types of cells under certain conditions in culture. Previously, we reported that a type I collagen matrix induced the osteoblastic differentiation of rat bone marrow stromal cells in culture. In this study, we performed a three dimensional culture within type I collagen gel to elucidate the relationship between the extracellular matrix of the cell culture system and the osteoblastic differentiation of rat bone marrow stromal cells.<BR>We compared the osteoblastic gene expressions of the culture cells; (i) culture in conventional culture dish (2D group), (ii) culture in type I collagen-coated culture dish (COAT group), and (iii) culture in three-dimensional type I collagen gel (3D group). In the 3D group of cell culture, the alkaline phosphatase activities of cells were higher than those of the other groups over 3 weeks of culture. Moreover, bone sialoprotein and osteocalcin mRNA expressions were found to be induced by the three-dimensional type I collagen gel culture of rat bone marrow stromal cells. These results suggested that the three dimensional culture of rat bone marrow cells is a crucial factor for inducing osteoblastic differentiation.
歯科基礎医学会の論文