Morphological changes in granular convoluted tubules and acini of mouse submandibular gland after methoxamine application. Histochemical properties and three-dimensional structure.:Histochemical properties and three-dimensional structure

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The present study was performed to investigate the morphological changes that occur in thegranular convoluted tubule and acinar cells of the mouse submandibular gland after the injection ofmethoxamine solutions into the abdominal cavity of male ICR mice.<BR>When a highly concentrated solution of methoxamine was given, the number of secretory granulesdischarged from the granular convoluted tubule cells increased and the time required for secretion wasreduced. As discharge increased, several vacuoles and dense granular-structures appeared in the cytoplasm. The three-dimensional structure obtained through computer analysis showed that a large majority of thevacuoles opened into the lumen of granular convoluted tubules. Therefore, the vacuoles seemed to haveoriginated from the limiting membranes of secretory granules after exocytosis.<BR>Positive reactions to Peanut agglutinin (PNA) and Soybean agglutinin (SBA), which did not occur in thelimiting membranes of secretory granules before exocytosis, were observed in membranes of the vacuoles. Deposition of PNA and SBA in the vacuoles may indicate the presence of sugar residues, which were maskedin the secretory granules before their contents were released.<BR>Lectin staining and transmission electron microscopy revealed that the dense granular-structures corresponded to secondary lysosomes breaking down the retrieved membranes.
歯科基礎医学会の論文