Characterization of secretory granular membranes from rat parotid gland: analysis of membrane lipid composition and fluidity.
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概要
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Membrane phospholipid compositions of a secretory granule-rich fraction (SG) from rat parotid glands were analyzed and compared with those of a microsomal fraction (Ms) and homogenates. Remarkable differences were concentrated in their phospholipid composition rather than their side-chain composition. SG had significantly higher levels of lysophospholipids (lyso-PL, 8.3 %) and phosphatidylethanolamine (PE, 30.5 %), whereas it had lower levels of phosphatidylcholine (PC. 40.3%) and phosphatidylserine (PS, 2.4%) than those of Ms. Although these phospholipids showed a characteristic side-chain composition depending on each phospholipid class, few differences among the three fractions were found in individual phospholipid side-chain compositions. Only PE from SG was relatively poor in the alkenyl chain, especially the octadec-1'-enyl chain, and concomitantly rich in octadecanoyl chain compared with PE from Ms. The side-chain compositions of total phospholipids from SG, Ms and homogenates were nearly similar to one another. Furthermore, ESR analysis demonstrated that fluidity of hydrophilic region of liposomes prepared by the phospholipids which were extracted from individual fractions, increase in the order of Ms<homogenates<SG. On the contrary, there were no differences in the fluidity of their hydrophobic regions.<BR>These results suggest that the granular membranes have a characteristic phospholipid profile, which affects the physical property of granular membranes.
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