Comparison of cell membrane proteins from gram-negative rods related to periodontitis.
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Cell membrane proteins of Gram-negative rods solubilized with sodium lauryl sarcosinate (Sarkosyl) were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The patterns obtained from the species of Bacteroides were different each other, and that was also so in Fusobacterium. Two strains of <I>B. asaccharolyticus</I>, three strains of <I>F. nucleatum</I> and two strains of <I>F. varium</I> showed different electrophoretic patterns. On the other hand, the patterns from <I>H. actinomycetemcomitans</I> and <I>C. ochracea</I> were similar within each strain, the pattern from <I>Bacteroides asaccharolyticus</I> ATCC 25260 was found to be similar to that from <I>C. ochracea</I>, however. These results indicate that it is difficult to utilize this method to identify the Bacteroides, Fusobacterium and <I>C. ochracea stralns.</I>
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