The arteries and veins in rat incisor pulp by TEM of thin sections and freeze-fracture replicas.
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The arteries and the veins in the rat incisor central pulp were studied by transmission electron microscopy of thin sections and the examination of freeze-fracture replicas. In thin sections, the central arteries, which were lined up in the median plane of the pulp, were classified as the arterioles according to their diameter. They were composed of three layers, that is, the intima which included endothelium and basal lamina, the media which included 2 to 4 layers of smooth muscle cells and the adventitia which was composed of loose connective tissue. The media was 3 to 4 μm in thickness. The central veins lay on both sides of the central arteries. They were classified as venules, however they were larger in diameter than normal venules. They were composed of endothelium, basal lamina and loose connective tissue. There were no smooth muscle cells in the wall of the veins in the pulp. In freeze-fracture replicas, <I>en</I> <I>face</I> views were obtained of the junctions between adjacent endothelial cells of the arteries and the veins. The junctions of the former were tight junctions constituted of 2 to 5 strands with many intercalated gap junctions. The junctions of the latter were 2 to 4 strands of linear creases (discontinuous low-profile ridges and grooves).
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