Establishment of EGF receptor-bearing cell lines by fusion between hamster embryonic palatal cells and mouse Ltk- cells.

概要

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Hamster embryonic palatal cells, which have functional EGF receptors but a limited life-span, were fused with mouse Ltk-cells, which bear no surface EGF receptors but have an infinite life-span. After HAT selection and cloning, the following three types of cell lines were obtained: i) clones bearing EGF receptors and having a proliferative response to EGF, ii) clones having EGF receptors, but unresponsive to EGF, and iii) clones bearing no EGF receptors and having no proliferative response to EGF. These cells provide appropriate materials for investigations into the role of EGF-receptors in the induction of cell proliferation by EGF. Cells of the first type will be a good source of immunogen for the production of antibodies against embryonic EGF receptors, which will be useful for the examination of the role of EGF in palatogenesis.
歯科基礎医学会の論文