Studies on electrolytes and water transport mechanisms in rat submandibular gland. The effects of ouabain, ethacrynic acid and furosemide.:The effects of ouabain, ethacrynic acid and furosemide

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In order to clarify some of the problems associated with the transport mechanisms of water and electrolyte especially in the basal and apical membranes of acinar portion of salivary gland, salivary flow rates and electrolyte concentrations of the rat submandibular glands were investigated by means of partial perfusions and intraductal injections of ouabain, ethacrynic acid and furosemide. The Na+, K+-ATPase activities in the salivary glands were also examined biochemically and histochemically according to the methods of JøUrgensen, and of Guth and Albers respectively. The following results were obtained. 1. Na+-K+ exchange pump of the basal membrane of acinar cell was inhibited by ouabain, ethacrynic acid and furosemide, respectively. Particularly, the inhibitory effect of ouabain was remarkable among these inhibitors. 2. It was assumed that there were inward sodium transport mechanisms exhibiting a specific sensitivity for ethacrynic acid and furosemide at the basal portion of the acinar cells. 3. The functional mechanisms of the Na+-K+ pump at the luminal membrane were significantly weak as compared with the Na+-K+ pump of basal portion. 4.It was suggested that ouabain inhibited Na+, K+-ATPase activity at the basal membrane of acinar cells, resulting in decrease of salivary flow rate.
歯科基礎医学会の論文