Primary culture of mouse submandibular gland epithelial cells embedded in collagen gel matrix.
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概要
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Salivary epithelial cells were isolated by collagenase digestion of male mouse (C57BL/6N) submandibular gland. Isolated cells (MSG cells) were embedded in the collagen (Type I) gel matrix. MSG cells demonstrated three-dimensional outgrowth with duct-like structures extending into the matrix. Growth of the cells reached 30-fold of that at plating after 3 weeks in culture, whereas fibroblasts did not grow in the matrix. In addition, MSG cells showed significant activity of amylase secretion into the culture medium even after 3 weeks in culture. Thus, MSG cells retained not only growth but differentiation when cultured in the collagen gel matrix. It is suggested that culture of MSG cells in the collagen gel matrix is a useful technique to study the effects of a variety of hormones and growth factors on the growth and differentiation of MSG cells.
- 歯科基礎医学会の論文
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