Hydrophobicity of the cell-surface of Streptococcus mutans.
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概要
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The hydrophobicity of cell-surface of <I>Streptococcus mutans</I> was studied using 11 laboratory strains of <I>S. mutans</I> and 6 strains of the mutant of <I>S. mutans</I> Kl-R. The hydrophobicity was measured by the adsorption of the microorganisms to hexadecane, and expressed as percent reduction from the initial optical density of the cell suspension. The hydrophobicity of laboratory strains of <I>S. mutans</I> varied from 45.9% to 98.0% in 0.01M potassium phosphate buffer (KPB), pH 7.2 and from 63.8% to 96.9% in PUM buffer (Rosenberg, <I>et al</I>., 1980). <I>S. mutans</I> AHT, 6715 and MT3940 showed higher values of the hydrophobicity in PUM buffer than in KPB. Mutants showed 64.9 to 100.7 percent of hydrophobicity of the parent in KPB. The hydrophobicities of the mutants as well as parent varied slightly between KPB and PUM buffer. The effect of cell density and pH on the hydrophobicity of <I>S. mutans</I> OMZ 176 (middle hydrophobic strain) was greater than that of <I>S. mutans</I> HS-6 (high hydrophobic strain). Furthermore washed cells of <I>S. mutans</I> HS-6, OMZ176, K1-R and mutant M38 were treated with subtilisin, trypsin, pepsin, dextranase, 5M of urea or heating at 100°C in order to study the change in hydrophobicity. Proteolytic enzymes produced decreasing hydrophobicity of all strains.<BR>The results of this experiment suggest that the hydrophobicity of cell-surface varies with strains of <I>S. mutans</I> and that the proteinic substance of the cell-surface is related to hydrophobicity.
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