Immunohistochemical studies in granular convoluted tubules of mice submandibular glands treated with secretagogues.
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The correlation between histological changes of secretory granules in granular convoluted tubule (GCT) cells, immunohistochemically detected EGF and NGF and binding patterns of PNA (Dgalactose binding lectin) and SBA (N-acetyl-D-galactosamine binding lectin) lectins were examined in mice submandibular gland under secretagogues administration. Morphometry of GCT in tissue sections and EGF contents by RIA were examined and compared to histologic and histochemical features.<BR>The secretion from GCT cells started immediately after administration of phenylephrine and methoxamine, and maximum degranulation in GCT cells was seen at 6 hours after administration. Accumulation of small sized secretory granules appeared in apical cytoplasms of GCT cells as a recovery sign at 12 hours after the administration.<BR>The GCT cell seemed to be mainly regulated by a-receptor. Effects of β-receptor may extend to the secretion of GCT since degranulation accompanied by reduced stainings of EGF, NGF and PNA, SBA lectins occurred after administration of isoproterenol. It was suggested that obvious correlation should be present between such changes of secretory granules and staining of EGF, NGF and PNA, SBA lectins.<BR>The PIA-EGF concentrations revealed an approximate 25% decrease in the male, 60% in the female from 1 to 6 hours after a-stimulation. Sexual dimorphism could be demonstrated objectively by the use of morphometry. The GCT area to the total was 45.8±4.08% in the male gland, 8.3±2.16% in the female. In the male, the value quickly decreased after a-stimulation and the lowest was 22.0±2.02% at 6 hours after administration.
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