Comparative study of various methods for identification of osteoid matrix in decalcified bone.

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Various methods for identification of osteoid matrix in decalcified bone were comparatively examined, using autoptic bone tissues from monkey and human. The osteoid volume in their sections measured morphometrically was also compared with that in the undecalcified control sections from the same materials.<BR>Both methods of Meyer, and Ralis and Ralis were very easy to handle, since decalcified sections can be prepared without special pretreatment of bone tissue and then stained in routine manner. They were however extremely unreliable for identification of osteoid matrix. Tripp and MacKay method which is based on von Kossa staining consists of incubation of bone tissue in a silver nitrate solution before decalcification, followed by decalcification and sectioning of the tissue. They showed clear identification of osteoid matrix, but the section made by this method sometimes brought about the remaining of the region without silver precipitation at the center of the calcified trabeculae, confusing it with the osteoid matrix. Yoshiki method consists of cyanuric chloride treatment of bone tissue before decalcification, then decalcification and sectioning of the tissue. It could easily obtain large decalcified sections from any bone tissues in routine manner and yielded high quality results for identification of osteoid matrix in the sections, resulting in easy application in any laboratory.
歯科基礎医学会の論文