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Changes in the secretory proteins of the rat submandibular saliva and its amino acid composition, brought about by a single dose of the parasympathomimetic drug, Pilocarpine, were studied. After collecting unstimulated saliva, the secretion was elicited by Pilocarpine and the saliva was collected at 10-minute intervals. Unstimulated saliva could be separated into seventeen components by SDS-electrophoresis, the stimuli were separated into twenty-six components both at 1 hr and 2hr. The carbohydrate-protein complex of stimulated saliva observed eleven components in contrast with unstimulated saliva which had eight components. Both unstimulated and stimulated saliva were consisted of aspartic acid, glutamic acid and proline-rich residues and with a given stimuli, the amino acid composition had a tendency to increase the basic amino acid residues. The low molecular bands in electrophoretogram contained in the cytosol fraction of the submandibular gland were absent from stimulated cytosol at 1 hr and 2hr, and Pilocarpine administration was effective in increasing the high molecule proteins in the cytosol at 1hr and 2hr. These results suggested that Pilocarpine brings about the discharge of salivary granule components into saliva and the differences in the secretory components with Pilocarpine stimuli and unstimuli, were evidenced.
歯科基礎医学会の論文