:IV Preparation of a chemically defined medium for the production of lytic enzyme from <I>Streptococcus mutans</I> AL7-1 strain

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Preparation of a chemically defined medium suitable for the production of lytic enzyme from <I>Streptococcus mutans</I> AL 7-1 strain was investigated. L-cysteine, vitamins and sodium carbonate, especially for this strain, were essential for the growth of this organism. In a synthetic medium containing twenty-one kinds of amino acids, glucose, vitamins and several kinds of salts, this strainwas cultured to produce the lytic enzyme. Up to a given time, the amount of lytic enzyme activities increased following its growth as more glucose was added, but after this time, a decrease was seen brought about by a lowering of pH of the culture medium in acc ordance with the acid production of this organism. As a result of statistical examinations of the factors interacting on the amount of thel ytic enzyme activities and its stabilities in the medium, the most suitable condition was seen when the initial pH of the medium containing 0.5% of glucose and 0.08 mole of phosphates was 7.0, andthe culture period was six hours. The preparations by fractionation on a Sephadex G 75 column of thel ytic enzyme from a chemically defined medium were not contaminated with the components of themedium, and showed a value of approximately 1.9 times the protein contents and ten fold rel ative acti-vities as compared with that of an organic medium.
歯科基礎医学会の論文