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L-ascorbic acid decalcification was used for electron microscopy of mammalian tooth germs. Immature enamel and dentine decalcified in 1-ascorbic acid were compared with specimens obtained with EDTA and the features of the ascorbic acid decalcification method studied. L-ascorbic acid decalcifies too h germs from dogs in less than 1/4-1/5 of the time it takes with the EDTA method. It preserves the organic matrix of enamel and dentine as well as in EDTA. Especially, enamel matrix was preserved better than in EDTA. These are two notable advantages of the ascorbic acid decalcification method. The rapid decalcification can raise the efficiency of experiments, it may prevent the dissolution of some compounds from the specimen and allow only minimal changes in the specimens during decalcification. The good preservation of organic matrix of the enamel may be useful for developmental studies of the tooth enamel. It is suggested that this L-ascorbic acid decalcification method can be put into practice for further studies of tooth enamel, in parallel with the conventional EDTA method.
歯科基礎医学会の論文