タイトル無し
スポンサーリンク
概要
- 論文の詳細を見る
The dissected incisor tooth germ in the mandible of a Wistar male rat, weighing 100-120g, was cultured in a medium consisting of Ham F12 supplemented with 10 per cent fetal calf serum, 100μ/ml penicillin and 100μg/ml streptomycin. The explants placed on cover glass and epoxy resin slide were cultivated in a humidified incubator at an atmosphere of 5% CO<SUB>2 </SUB>in air and closed system at 37°C.<BR>The outgrowing cells were star-shaped and <I>migrated</I> in a pavement-like pattern, not separately. The cells were a mixed population of mesenchymal cells and epithelial cells originating from the incisor tooth germ. After one week of cultivation, a keratinized cell was observed by staining with Papanicolaou. A nest of epithelial cells was seen in the monolayer cell sheet after 3 weeks of cultivation, which enlarged gradually, but it shrank at 3 weeks after their fisrt appearance. Ultrastructually, mesenchymal cells during their early stage of cultivation were similar to undifferentiated fibroblast of the dental papilla of rat in vivo. They were characterized by a large ovoid nucleus, mitochondria <I>rER</I>, lysosome, small oil drops and several cytoplasmic processes. At a later stage, the mesenchymalcell was flattened on the glass surface, and included microfilament and 10 nm filament. The cultured epithelial cell included relatively more tonofilament than odontogenic epithelial cell of the tooth germ in vivo.<BR>The present method seems to be useful as a experimental system for analysis of the cellular function of fibroblast originating from dental papilla.
- 歯科基礎医学会の論文
歯科基礎医学会 | 論文
- 口腔レンサ球菌のベータディフェンシン感受性の多様性とその機構の解析
- ココアによる歯周病関連菌に対する抗菌効果
- Candida albicansに対するDefensinの抗菌活性の検討
- 唾液腺腺様嚢胞癌の偽嚢胞腔内の免疫グロブリン
- 破骨細胞が産生するcathepsin KとMMP-9の局在について : 肺癌骨転移モデルを用いた検討