タイトル無し

概要

論文の詳細を見る
Water-insoluble extracellular materials synthesized in vitro by Streptococcus mutans of serotype a to g was examined on two different dried specimens by scanning electron microscopy. Air-dried specimens revealed that serotype a to e grown in sucrose-containing Trypticase Soy broth produced sticky amorphous capsule-like extracellular polysaccharide and serotype f and g produced massive extracellular materials. Serotype e to g also produced the extracellular materials which seemed to play a role of adherence to smooth surface in sucrose-free broth. In contrast to air-dried specimens, ethanol-dehydrated specimens visualized relatively smooth cell surface of serotype a to d grown in sucrose broth. Filamentous net-like structures by which many cells were assembled together were observed in the ethanol-dehydrated specimens of serotype b and d. Similar net-like structures were also observed on the cell surface of serotype g grown in sucrosefree broth, whereas massive materials as well as net-like structures were observed in sucrose-containing broth. These findings suggest that air-dried specimens may be suitable for morphological observation of intact insoluble extracellular polysaccharide of S. mutans and that significant differences in morphology of the extracellular materials observed among S. mutans serotypes may reflect differences in extracellular polysaccharides which they produced.
歯科基礎医学会の論文