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The effects of hormones on biochemical functions in the parotid glands were studied <I>in vitro</I> using rat parotid explants which were supported by siliconized lens papers at the surface of the chemically defined synthetic medium.<BR>Isoproterenol (IPR) stimulated amylase secretion from the explants which had been cultured for 48 hours in Medium 199 and total amylase activity in the culture system increased gradually during the culture period. IPR also stimulated DNA synthesis in the presence or absence of insulin and dexamethasone. This stimulation was preceded by enhanced syntheses of RNA and protein and marked induction of ornithine decarboxylase (ODC). Similar effects on DNA synthesis and ODC activity were observed with epinephrine or norepinephrine but the responses were less pronounced than in those with IPR. DNA synthesis was also stimulated by insulin and dexamethasone. In this case, however, increases of RNA and protein synthesis and ODC activity in the prereplicative phase were not so remarkable as in the case of IPR. Dibutyryl cAMP raised ODC activity but was inhibitory on DNA synthesis.<BR>These results suggest that the rat parotid explants maintain hormonal responsiveness for at least 48 hours and can be used as experimental tools for study of physiology of the parotid glands.
歯科基礎医学会の論文