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The interspecific relationship between Gentiana scabra and G. triflora and their intra-specific variation were investigated by RAPD analysis using 15 strains composed of 4 G. scabra lines, 6 G. triflora lines, 3 interspecific hybrids and 2 intra-specific hybrids of G. triflora. Out of 36 primers used for RAPD analysis, 8 primers generated 53 polymorphic DNA fragments, among them. Quantification theory (Suryoka) III and cluster analysis based on the polymorphic data showed that these two species were divided into two separate groups except for one G. scabra strain, 'Oz' which belongs to the G. triflora group. All the hybrid lines between G. scabra and G. triflora could be classified into the G. scabra group. RAPD fragments specific to each species were cloned and sequenced. The sequences were further used to design SCAR (Sequenced Characterized Amplified Region) primer sets for discriminating between the two species. The SCAR primer set specific to G. scabra, amplified a specific fragment in all the strains of G. scabra except for the 'Oz' line, and the primer set specific to G. triflora gave a specific fragment in 6 out of 8 G. triflora strains and the intraspecific hybrids. These results indicated that the SCAR markers may be good indicators for discriminating between the two Gentiana species.
日本育種学会の論文