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A stripe resistance gene, <I>Stvb-i</I>, was introduced into <I>japonica</I> cultivars from an <I>indica</I> cultivar Modan in Japan. A set of primers for PCR assay was designed based on the sequence information of a RFLP marker ST10 which is linked by 0.0 cM to the <I>Stvb-i</I> gene. This set of primers, which generate a SCAR (sequence characterized amplified region) marker, was referred to as "ST10 primers" . In PCR with the ST10 primers, a 727-bp DNA fragment was amplified specifically from all the tested rice plants harboring the <I>Stvb-i</I> gene derived from Modan: resistant cultivars, lines and F<SUB>1</SUB> individuals from a cross of the susceptible parent 'Koganebare'/resistant parent 'Tsukinohikari'. On the other hand, the fragment was not amplified in susceptible cultivars. In 120 of the F2 individuals derived from the susceptible parent 'Koshihikari'/resistant parent 'Asanohikari', the resistant homozygous and heterozygous individuals showed a positive reaction in the PCR assay with the ST10 primers. The 727-bp fragment was identified as a part of the ST10 fragment (745 bp) by sequence analysis. In foreign cultivars, the ST10 primers amplified the 727-bp fragment in 3 out of 9 tested resistant cultivars that have been reported to harbor the same resistance gene, <I>Stvb-i</I>. The three cultivars were Modan, IR8 and Mudgo. The 727-bp fragment was not amplified in a foreign cultivar 'Zenith'and Japanese upland cultivars harboring other resistance genes, Stva and Stvb. The SCAR marker can be used in the markeraided selection of stripe resistant individuals carrying the <I>Stvb-i</I> gene derived from Modan. Therefore, screening for stripe resistance can now be conducted independently of the bioassay with vector insects.
- 日本育種学会の論文
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