Measurement of Micronuclei by Cytokinesis-Block Method in Buffalo Lymphocytes.
スポンサーリンク
概要
- 論文の詳細を見る
Cytokinesis-block micronucleus method was used to obtain a standardized quantitative assay for spontanous micronuclei in buffalo lymphocytes, as first report. Blood lymphocytes of buffalo from a clean environmental area were cultured to study the effect of different concentrations of cytochalasin B (cyt-B) on the frequencies of mononucleated, binucleated and polynucleated cells. The result showed that the optimal concentration of cyt-B to obtain the highest frequency of binucleated cells was 4μg/ml (126.8±17.0/1000 cells). The micronuclei (MN) frequency varied from 9 to 22 with a mean value of 13.5 ±2.9/1000 cells. A comparative study between the MN formation in buffalo lymphocytes from clean and polluted environmental areas revealed that the animals exposed to industrial emission of heavy metals (chromium, copper, zinc, cadmium, lead) showed a significant increase in MN formation 44.1±2.0/1000 cells compared to 13.5±2.9/1000 cells in buffalo from clean environmental area. Based on our results, cyt B-method can be applied as a reliable, simple and inexpensive method to assess mutagenic factors and hygienic level of environmental surroundings in buffalo.
- 財団法人 日本メンデル協会の論文
財団法人 日本メンデル協会 | 論文
- The Chromosomes of Dicranocephalus agilis (Hemiptera: Heteroptera)
- Consistently greater decondensation of the nuclear DNA-protein complexes from normal lymphocytes than from acute and chronic lymphocytic leukemia cells following treatment with disulfide reducing agents.
- The Reversion of Chromoplasts to Chloroplasts in Valencia Oranges
- Two Problems in the Interpretation of Meiosis in Plants
- Cytology of Trichosanthes palmata Roxb