Chromosomal Aberrations and Sister Chromatid Exchanges in Cultured Human Lymphocytes I. Induced by Crude Extracts of Black and Green Tea.
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Crude extracts of black and green tea (BT and GT) (Camellia sp.) were assayed for chromosomal aberration and sister chromatid exchanges (SCE's) inducing effects in cultured human lymphocyte cells. The concentrations used were 0.01, 0.1, 1.0 and 10.0μg/ml. Two types of treatments were performed:(1) Type 'A'-Cells were grown in presence of different concentrations of tea extracts.(2) Type 'B'-Precultured (for 24 hr) cells were treated for 3 hr in plain medium (without serum, PHA and BrdU) with different concentrations of tea extracts. After two washings, cells were resuspended in complete medium (containing serum, PHA and BrdU) and incubated for desired incubation period i. e. 48 and 72 hr for chromosomal aberration and SCE analysis, respectively.The percentage values of aberrant metaphases ranged from 3.33% to 6.0% and 0.0% to 5.0% due to respective treatments 'A' and "B" of BT and 2.0% to 7.0% and 1.0% to 4.0% after treatments 'A' and 'B' of GT respectively. Effects did not appear to be dose dependent. Chromatid gaps and breaks were commonly observed. Terminal deletions and chromosome gaps were observed only occasionally. Treatment 'A' and 'B' of BT enhanced the SCE frequency. Treatment 'A' of GT enhanced the SCE's but treatment 'B' decreased the SCE's which might be due to the induction of DNA repair process (es). Mutagenic and antimutagenic effects of tea have been discussed. From the present study it can be suggested that while tea may protect the human genome against certain environmental agents, the possibility can not be ruled out that alone or in combination of other co-factors it may pose genetic threat.
- 財団法人 日本メンデル協会の論文
財団法人 日本メンデル協会 | 論文
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