Nuclear Membrane and Cytoplasmic Lamellae at Meiotic Prophase of Lilium Microsporocytes

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The ultrastructure of nuclear membrane and cytoplasmic lamellae have been studied at meiotic prophase in the microsporocytes of Lilium in vivo and in vitro in presence and absence of mitomycin C. Meiocytes were fixed in glutaraldehyde and OsO4 and sections were stained in uranyl acetate and lead citrate. The nuclear pores have been found to be octagonal in shape with eight microcylinders. The pore complexes are probably detached from the nuclear wall during the breakdown of the latter. At diffused stage in the mitomycin treated meiocytes triple layered unit membranes have been seen to form cytoplasmic lamellae. Two structural components, small dense granules and exceedingly thin filaments, have been seen always to be associated with these lamellae. These lamellae stain basophilic. They later arrange into stacks and change over to tubular ellipsoid forms. Sometimes these threelayered unit membranes have been seen to dissociate from chromatin at the time of the breakdown of the nuclear wall. The significance of the formation of these cytoplasmic lamellae has been discussed.The author thanks Prof. J. Straub, Dr. I. Anton-Lamprecht for their interest in this study and to Mrs. S. Sen, Miss B. Meyer and Mr. D. Bock for help. Financial grants from Alexander von Humboldt Stiftung and MaxPlanck-Gesellschaft are acknowledged gratefully.
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