Alteration of nuclear transcription factors NF-.KAPPA.B and AP-1 with DNA binding activity in discrete brain structures of streptozotocin-induced diabetic rats with hyperglycemia.

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<I>De novo</I> protein synthesis in eukaryotes is mainly controlled at the level of gene transcription by transcription factors in the nucleus. In this communication, DNA binding activities of nuclear factor (NF) -κB and activator protein-1 (AP-1) were determined in the brain from streptozotocin (STZ) -injected diabetic rats in order to explore particular proteins related with diabetes. Gel retardation electrophoresis using core consensus elements containing NF-κB or AP-1 probe revealed that STZ significantly potertiated NF-κB binding in the cerebral cortex and medulla-pons. Moreover, STZ markedly decreased NF-κB binding in the striatum and hypothala-mus. However, no significant changes was detected in NF-κB binding in the hippocampus, midbrain and cerebellum. In contrast, STZ significantly potentiated AP-1 binding in the cerebellum and medulla-pons, whereas STZ markedly decreased AP-1 binding in the striatum, hypothalamus and midbrain. However, AP-1 binding was not markedly affected by the injection of STZ in the cerebral cortex and hippocampus. These results suggest that the STZ-injected diabetic rat shows differential alterations in DNA binding activities of transcription factors NF-κB and AP-1 in the brain.
日本歯科薬物療法学会の論文